Journal: Molecular neurobiology

Article Title: CD38 Coordinates with NF-κB to Promote Cochlear Inflammation in Noise-Induced Hearing Loss: the Protective Effect of Apigenin.

doi: 10.1007/s12035-024-04675-7

Figure Lengend Snippet: Fig. 9 CD38 coordinates with NF-κB to promote cochlear inflammation in NIHL. As the closest immune cell to organ of Corti, activated macrophages can express CD38 and consume NAD in organ of Corti, causing NAD + metabolism dysfunction and ultimately leads to cochlear inflammation. In this process, inflammatory factors such as IL-1, IL-6, and TNF-α pro- moted the activation of NF-κB signaling pathway, forming a positive feedback cycle

Article Snippet: Immunofluorescence The cochlear basilar membrane was prepared with the surface preparation method, antibodies used in this experiment were rabbit anti-NF-κB p65 polyclonal antibody (Abcam, ab32536), rat anti-F4/80 polyclonal antibody (Abcam, ab6640), and mouse anti-CD38 monoclonal antibody (Proteintech, 60,006–1-lg).

Techniques: Activation Assay

Journal: Cell communication and signaling : CCS

Article Title: Tumorous IRE1α facilitates CD8 + T cells-dependent anti-tumor immunity and improves immunotherapy efficacy in melanoma.

doi: 10.1186/s12964-024-01470-8

Figure Lengend Snippet: Fig. 2 Tumorous IRE1α facilitates anti-tumor immunity in a CD8+T cells-dependent manner. A Schema of the treatment in C57BL/6 mice bearing B16F10 tumors received HA15 treatment as indicated. Tumor burdens, weights and volumes in each group were calculated and displayed in B and C. D Representative flow cytometry data and summary plots of the frequency of CD3+CD45+, CD8+CD3+, CD11c+CD45+, Foxp3+CD4+ and CD8+T-cells evaluated for expression of Granzyme B and IFN-γ in tumor from xenografts with indicated treatment. E Immunofluorescence staining of XBP1s or CD8α in B16F10 xenografts with or without the treatment of HA15. Scale bar, 50 μm. F-H Scheme representing the experimental procedure (F), tumor burdens, tumor weight (G), and tumor volume (H) of C57BL/6 mice injected subcutaneously with B16F10 tumors with treatment of HA15 and αCD8 depleting antibodies either alone or in combination. Symbols of one dot indicates one mouse, and the error bars are mean with ± SD (n = 4). Two-tailed Student’s t-test (*p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant)

Article Snippet: Heat-mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) was performed, the sections were blocked with 10% goat serum in PBS for 1 h at room temperature and then stained overnight at 4 °C with rabbit pAb to XBP1s (1:100, 24868-1-AP, proteintech), rabbit pAb to Annexin A1 (1:50, 21990-1-AP, proteintech), rabbit pAb to Calreticulin (1:50, 27298-1-AP, proteintech), rabbit pAb to HMGB1 (1:50, 10829-1-AP, proteintech), rabbit mAb to p-MLKL (Ser345) (1:500, 37,333, CST), mouse mAb to Foxp3 (1:50, 65089-1-Ig, proteintech), CoraLite® Plus 488 Rat mAb to CD4 (1:50, CL488-65141, proteintech), rabbit pAb to Granzyme B (1:50, 13588-1- AP, proteintech), rabbit pAb to IFN-γ (1:50, 105,995-T08, Sinobiological) or rat mAb to CD8α (1:50, 65069-1-Ig, proteintech) antibody.

Techniques: Flow Cytometry, Expressing, Immunofluorescence, Staining, Injection, Two Tailed Test

Journal: Cell communication and signaling : CCS

Article Title: Tumorous IRE1α facilitates CD8 + T cells-dependent anti-tumor immunity and improves immunotherapy efficacy in melanoma.

doi: 10.1186/s12964-024-01470-8

Figure Lengend Snippet: Fig. 6 ER stress inducer enhances the anti-tumor activity of anti-PD-1 antibody in vivo. A Schema of the treatment in C57BL/6 mice bearing B16F10 tumors received HA15 with or without anti-PD-1 antibody combination treatment as indicated. B Images of isolated tumors from mice that received indicated treatment. Tumor volumes and weights in each group were calculated and displayed in C and D. E-H Representative flow cytometry data and summary plots of the frequency of CD3+CD45+, CD8+CD3+, F4/80+CD11b+CD45+, Foxp3+CD25+CD4+ and CD8+T-cells evaluated for expression of Granzyme B and IFN-γ in tumor from xenografts with indicated treatment. Symbols of one dot indicates one mouse, and the error bars are mean with ± S.D (n = 4). Two-tailed Student’s t-test (*p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant)

Article Snippet: Heat-mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) was performed, the sections were blocked with 10% goat serum in PBS for 1 h at room temperature and then stained overnight at 4 °C with rabbit pAb to XBP1s (1:100, 24868-1-AP, proteintech), rabbit pAb to Annexin A1 (1:50, 21990-1-AP, proteintech), rabbit pAb to Calreticulin (1:50, 27298-1-AP, proteintech), rabbit pAb to HMGB1 (1:50, 10829-1-AP, proteintech), rabbit mAb to p-MLKL (Ser345) (1:500, 37,333, CST), mouse mAb to Foxp3 (1:50, 65089-1-Ig, proteintech), CoraLite® Plus 488 Rat mAb to CD4 (1:50, CL488-65141, proteintech), rabbit pAb to Granzyme B (1:50, 13588-1- AP, proteintech), rabbit pAb to IFN-γ (1:50, 105,995-T08, Sinobiological) or rat mAb to CD8α (1:50, 65069-1-Ig, proteintech) antibody.

Techniques: Activity Assay, In Vivo, Isolation, Flow Cytometry, Expressing, Two Tailed Test